It takes FASTA DNA sequence as input, and write GFF3 as output. It uses the new NHMMER tool that comes with HMMER 3.1 for HMM searching in RNA:DNA style. NHMMER binaries for 64-bit Linux and Mac OS X are included and will be auto-detected. Multithreading is supported and one can expect roughly linear speed-ups with more CPUs. Mac OS X:/Applications/Blast; Windows: C: Blast. 2 Extract the archives of BLAST. Copy the blast installation file for your platform from the aqp-tutorial-files blast-install directory to the directory you've made. In Unix or Linux, extract the files by using the command tar zxvf filename. On Mac OS X or Windows, double-click the file. Centrifuge is a very rapid and memory-efficient system for the classification of DNA sequences from microbial samples, with better sensitivity than and comparable accuracy to other leading systems. The system uses a novel indexing scheme based on the Burrows-Wheeler transform (BWT) and the Ferragina-Manzini (FM) index, optimized specifically for the metagenomic classification problem.